№ 768
F polymerase
Description
High-processional and high-precise DNA polymerase, having high resistance to inhibiting.
Enzyme suits for paragraphs amplification till 20 kb and with higher speed and accuracy. So, for amplification of 1 kb elongation duration equals to 15-30 sec only. F polymerase can be used for carrying out of mutagenesis and creating of gene libraries.
Scopes of application:
• High-precise PCR;
• Long-range PCR (>10 kbp);
• «Direct-PCR» with using of EDTA-stabilized blood (up to 15%) as a matrix
Enzyme characteristics:
• exhibits 5’→3’ polymerase activity;
• has corrective 3’→5’ exonucleolytic activity, providing high precision of amplification (in 50 times more precise than Taq polymerases).
• 5’→3’ exonucleolytic activity is absent
• Gives high yield of amplification products
• Is resistant to presence of inhibitors: up to 15% of whole blood can be added directly to PCR.
• allows to receive blunt-ended DNA, i.e. it doesn’t suit for A/T cloning
• Doesn’t have hot-start