№ 442-01

Urogenital trichomoniasis is a sexually transmitted infection, the causative agent of which is the simplest unicellular parasite – Trichomonas vaginalis. In the structure of all STDs, trichomoniasis is one of the first places and leads in terms of the frequency of detection in persons who have applied for specialized dermatovenereological, obstetric-gynecological and urological help for infectious and inflammatory diseases of the urogenital tract.

Infection of adult men and women occurs during sexual contact, children - through the passage of the birth canal of a sick mother, sexual contact or contact and domestic way.

Qualitative determination of  Trichomonas vaginalis DNA in the scrapedoff mucous membranes of the urogenital tract, urine samples, secretion of the prostate and other biological materials by the PCR method with hybridization-fluorescent detection in real time.

To increase the sensitivity and specificity of the analysis, Taq polymerases with «hot start» are used, and the inclusion of uracil-DNA glycosylase (UDG) and deoxyuridine triphosphate (dUTP) in the reaction mixture reduces the risk of cross contamination.