№ 753

Uracil-DNA-glycosylase (UDG)

PCR

Reagents for PCR

Description

An enzyme that catalyzes the release of uracil from uracil-containing single- or double-stranded DNA, but does not affect dTTP. The resulting abasic site blocks the work of DNA polymerases and undergoes hydrolytic cleavage at elevated temperature and high
pH. Enzyme itself is also sensitive to heat and can be inactivated at temperatures above 70°C.

UDG can be used in the system of combating contamination with products of previously conducted PCR, which reduces the risk of false positive results