Alkor Bio Group is to expand its portfolio of oligonucleotides for external customers
In mid-May, Alkor Bio Group of Companies, a developer and manufacturer of laboratory diagnostic reagent kits, announced a significant expansion of the range of oligonucleotides to offer to external customers. They include both unmodified and modified oligonucleotides and different of probes for real-time PCR analysis.
Leader of the oligosynthesis group in the molecular diagnostics laboratory Alexandra Perchik says:
“We started production of different types of oligonucleotides for our internal needs (research and development, manufacturing of PCR and microarray-based diagnostics kits) in 2012. In the process, we managed to optimize our practices and achieve high quality, and in 2013 we began to offer oligonucleotide synthesis services for external customers. Currently we can offer 23 different modifications of oligonucleotides, including recently introduced LNA, Alkyne, Cy3, Cy3,5, Cy5,5 modifications and oligonucleotide probes Cy3-BHQ2, Cy3,5-BHQ2, Cy5,5-BHQ2.”
Recently company invested 10 million roubles (150 000 USD) in acquisition of high-performance MerMade 48X oligonucleotide synthesizer (BioAutomation, USA). With capacity of up to 100 oligonucleotides a day, it helps to meet growing demands from both internal and external customers. All oligonucleotides undergo purification and quality control steps to meet necessary requirements for different molecular biology applications.
Oligonucleotides are short fragments of nucleic acids with a given sequence, chemically synthesized from special reagents on automatic synthesizers. They are widely used in different molecular biology research and biomedical applications, e.g. PCR, microarrays, sequencing, gene synthesis and many others. They also can contain different molecules (modifications) that give them certain properties. For example, oligonucleotide probes for real-time PCR contain fluorophore label molecule and “quencher” molecule that suppresses the fluorescence; during reaction, suppression removed and process of amplification detected by fluorescent signal.