Due to the global spread of the new Omicron variant and other mutations, Alkor Bio specialists promptly analyzed their impact on the detection of SARS-CoV-2 RNA using the "Intifica SARS-CoV-2" reagent kit.  The following strains occurring in the Russian Federation were examined by in vitro method:

• British (VOC Alpha 202012/01 GRY (B.1.1.7))
•  Brazilian (VOC Gamma GR/501Y.V3 (P.1))
•  Indian (VOC Delta G/478K.V1 (B.1.617.2))
•  Moscow (B.1.1.523)- Nigerian (VOI Eta GR/484K.V3 (B.1.525))
•  Northwest (AT.1)

As a result of the study, it was indicated that mutations in these strains do not affect RNA detection of the virus, and the Intifica SARS-CoV-2 reagent kit accurately detects all three virus-specific targets. In addition, we analyzed an in silico to assess the possible impact of mutations of different strains of the SARS-CoV-2 virus that are widespread in other countries and may be detected in the Russian Federation:

• South African (VOC Beta GH/501Y.V2 (B.1.351))
• California (VOI Epsilon GH/452R.V1, (B.1.429+B.1.427/CAL.20C))
•  Brazilian (VOI Zeta GR/484K.V2 (P.2))
•  Philippine (VOI Theta GR/1092K.V1 (P.3))
• American (VOI Iota GH/253G.V1 (B.1.526))
• Indian (VOI Kappa G/452R.V3 (B.1.617.1))
• Peruvian (VOI Lambda GR/452Q.V1 (C.37))
• Colombian (VOI Mu GH (B.1.621+B.1.621.1))
•  Botswana (VOC Omicron GR/484A (B.1.1.529))

This analysis showed that mutations in these strains do not affect the target regions of the virus genome detected by the SARS-CoV-2 Intifica reagent kit.